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| Mori, Fumiki; Nakamura, Tadashi; Nakamura Osawa, Aya; Arai, Ikichi; Urashima, Tadasu; 森, 史樹; 中村, 正; 男澤－中村, 綾; 荒井, 威吉; 浦島, 匡. |
| Cell wall polysaccharides were separated from Leuconostoc mesenteroides subsp. cremoris isolated from “Filmjolk"，a fermented milk made commercially in Sweden. Two Components(CNPI and CNP2) were further purified by gelfiltration on HPLC and the molecu1ar weights were determined to be 850，000 and 81，000. CNP-2 was shown to contain two polysaccharides in major and minor by an lH-NMR. The major polysaccharide was shown to have a heptasaccharide repeating unit which contained five α and two β anomers，and also contained non reducing galactopyranose，O-2，3 disubstituted glucopyranose，O-3 substituted galactopyranose and O-3，6 disubstituted galactopyranose as units in a molar ratio of 3:1:1:2. On the other hand，the minor polysaccharide was shown to have a... |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2921 |
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| 荒井, 威吉; 仙田, 晶嗣; 山上, 貴礼; 無量谷, 亜希; 中村, 正; 浦島, 匡; ARAI, lkichi; SENDA, Akitsugu; YAMAGAMI, Kihiro; MURYOYA, Aki; NAKAMURA, Tadashi; URASHIMA, Tadasu. |
| The efficacy of the use of acidic electrolyzed water (AEW) for premilking teat cleaning and disinfection (TCD) was evaluated using a split-udder experimental design. The efficacy of AEW for TCD was compared with that of NaClO solution contained 200 ppm available chlorine (HClO). The viable cell counts levels of SPC，lactic acid bacteria，thermotrophilic bacteria and anaerobe (group A) which contaminated on teat skin were over 10³ cfu/cm²，whereas psychrotrophilic bacteria and coliforms (group B) were less 10² cfu/cm². After TCD with AEW and HClO，viable cell counts levels of the microorganisms of group A and group B were decreased to 2.8-7.6% and 10.0-14.5% respectively. On the other hand，the efficacy of AEW or HClO for TCD was almost constant throughout one... |
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Palavras-chave: 強酸性電解水; 乳頭清拭; Acidic electrolyzed water; Premilking teat cleaning and disinfection. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2993 |
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| 仙田, 晶嗣; 中村, 正; 浦島, 匡; 荒井, 威吉. |
| 生乳中の酵母の動態を明らかにすることを目的として，帯広畜産大学と近郊の酪農家3戸で飼養している健康なホルスタイン種乳牛（体細胞数1×105cfu/ml以下）の乳頭表皮，分房乳，バルク乳およびローリー乳中の酵母数の月別変動について調べた．各試料は1999年7月から2000年6月までの1年間，それぞれ毎月1回ずつ採取した．生乳中の酵母の検出率と平均酵母数はそれぞれ順に，乳頭表皮では70.1％，52cfu/cm2，分房乳は47.9％，1.8cfu/ml，バルク乳は100％，28cfu/mlおよびローリー乳では100％，66cfu/mlであった．乳頭表皮および各種類の生乳中において90％以上を占める酵母数の水準は，乳頭表皮が150cfu/cm2以下（90.8％），分房乳が10cfu/ml以下（94.4％），バルク乳が100cfu/ml以下（97.8％）およびローリー乳が150cfu/ml以下（91.7％）であると考えられた．生乳中の酵母数は夏から秋期に高く，冬から春期に低くなる季節的な変動傾向が認められた．健康な乳牛の分房乳中では，主な乳房炎起因菌（C. parapsilosis, C. lambica, C. catenulata, C. rugosa）は夏期に検出されたが，冬から春期には検出されなかった． The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/ml) in a high quality raw milk produced by healthy Holstein cows (≤1×105 somatic... |
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Palavras-chave: Yeast in milk; The incidence of yeast; The seasonal changes of yeast counts. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2640 |
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| Sumiyoshi, Wataru; Urashima, Tadasu; Nakamura, Tadashi; Arai, Ikichi; Nagasawa, Takashi; Saito, Tadao; Tsumura, Norihiko; Wang, Bing; Brand-Miller, Janette; Watanabe, Yoko; Kimura, Kazumasa; 住吉, 渉; 浦島, 匡; 中村, 正; 荒井, 威吉; 長澤, 孝志; 齋藤, 忠夫; 津村, 宣彦; 渡邉, 陽子; 木村, 一雅. |
| The concentrations of the six major sialyl oligosaccharides found in the milk of 24 Japanese women were determined using reverse phase high performance liquid chromatography of the 3-methyl-1-phenyl-5- pyrazolone derivatives of the oligosaccharides. The milk was collected at 4, 10, 30 and 100 days postpartum. The concentrations of all six oligosaccharides decreased during the course of lactation. The concentrations of 6'-sialyllactose, sialyllacto-N-tetraose b and disialyllacto-N-tetraose decreased only after 10 days postpartum, whereas those of sialyllacto-N-tetraose a and sialyllacto-N-neotetraose c as well as 3'-sialyllactose decreased from 4 days postpartum. Large differences were observed between present data and those previously reported for Italian... |
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Palavras-chave: Human milk; Sialyl oligosaccharide; Lactation period; PMP; Reverse phase HPLC. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3287 |
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| 荒井, 威吉; 中島, 景典; 丸山, 千弘玲; 中村, 正; 戸羽, 隆宏; 浦島, 匡; Arai, Ikichi; Nakajima, Keisuke; Maruyama, Chigure; Nakamura, Tadashi; Toba, Takahiro; Urashima, Tadasu. |
| Microflora of lactic acid bacteria and yeasts of Laban which had been made with bovine or ovine milk by traditional natural fermentation in Yemen，were studied. The mean of viable cell counts of microorganisms in Laban was 6.4 x10⁸ cfu/ml. The 60 strains of lactic acid bacteria and 24 strains of yeasts were isolated and identified. The percentages of strains on lactic acid bacteria flora were as follows: Lactococcus lactis subsp.lactis① was dominant in 88%. Lc. lactis subsp. lactis②，Leuconostoc mesenteroides subsp. mesenteroides，and Leuc. lactis were in 5，5，and 2%，respectively. It was recognized that the some characteristics of Lc. lactis subsp. lactis① of the dominant strain were different from those of Lc. lactis subsp.lactis②. The percentages of strains... |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2923 |
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| 中村, 正; 菅井, 理子; 男澤, 綾; 有賀, 秀子; 小疇, 浩; キュラ, キーユキア; 荒井, 威吉; 浦島, 匡; Nakamura, Tadashi; Sugai, Michiko; Nakamura, Aya; Ariga, Hideko; Koaze, Hiroshi; Kiiyukia, Cura; Arai, Ikichi; Urashima, Tadasu. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1877 |
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